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Article Title: The crystal structure of Pseudomonas aeruginosa lipoxygenase Ala420Gly mutant explains the improved oxygen affinity and the altered reaction specificity.
Article Snippet: The crystal structure of Pseudomonas aeruginosa lipoxygenase Ala420Gly mutant explains the improved oxygen affinity and the altered reaction specificity Jacqueline Kalms, Swathi Banthiya, Etienne Galemou Yoga, Mats Hamberg, Hermann-Georg Holzhutter, Hartmut Kuhn, Patrick Scheerer PII: S1388-1981(17)30003-3 DOI: doi:10.1016/j.bbalip.2017.01.003 Reference: BBAMCB 58101 To appear in: BBA - Molecular and Cell Biology of Lipids Received date: 25 October 2016 Revised date: 3 January 2017 Accepted date: 12 January 2017 Please cite this article as: Jacqueline Kalms, Swathi Banthiya, Etienne Galemou Yoga, Mats Hamberg, Hermann-Georg Holzhutter, Hartmut Kuhn, Patrick Scheerer, The crystal structure of Pseudomonas aeruginosa lipoxygenase Ala420Gly mutant explains the improved oxygen affinity and the altered reaction specificity, BBA - Molecular and Cell Biology of Lipids (2017), doi:10.1016/j.bbalip.2017.01.003 This is a PDF file of an unedited manuscript that has been accepted for publication.. As a service to our customers we are providing this early version of the manuscript.. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form.

Article Title: Crystal Structure of Arachidonic Acid Bound to a Mutant of Prostaglandin Endoperoxide H Synthase-1 That Forms Predominantly 11-Hydroperoxyeicosatetraenoic Acid
Article Snippet: Materials—Arachidonic acid, 11R-HETE, and 15S-HETE were pur- chased from Cayman Chemical Co. (Ann Arbor, MI).

Article Title: Synergistic effect of 15-lipoxygenase 2 and radiation in killing head-and-neck cancer.
Article Snippet: 15S-HETE and AA were purchased from Cayman Chemical Inc. (An Arbor, MI).

Article Title: Strata movement and stress evolution when mining two overlapping panels affected by hard stratum
Article Snippet: 15s-HpETE and 15s-HETE were purchased from Cayman Chemical (Ann Arbor, MI, USA).

Article Title: Structural and functional basis of phospholipid oxygenase activity of bacterial lipoxygenase from Pseudomonas aeruginosa.
Article Snippet: Structural and functional basis of phospholipid oxygenase activity of bacterial lipoxygenase from Pseudomonas aeruginosa Swathi Banthiya, Jacqueline Kalms, Etienne Galemou Yoga, Igor Ivanov, Xavi Carpena, Mats Hamberg, Hartmut Kuhn, Patrick Scheerer PII: S1388-1981(16)30222-0 DOI: doi: 10.1016/j.bbalip.2016.08.002 Reference: BBAMCB 58023 To appear in: BBA - Molecular and Cell Biology of Lipids Received date: 19 May 2016 Revised date: 29 July 2016 Accepted date: 3 August 2016 Please cite this article as: Swathi Banthiya, Jacqueline Kalms, Etienne Galemou Yoga, Igor Ivanov, Xavi Carpena, Mats Hamberg, Hartmut Kuhn, Patrick Scheerer, Structural and functional basis of phospholipid oxygenase activity of bacterial lipoxygenase from Pseudomonas aeruginosa, BBA - Molecular and Cell Biology of Lipids (2016), doi: 10.1016/j.bbalip.2016.08.002 This is a PDF file of an unedited manuscript that has been accepted for publication.. As a service to our customers we are providing this early version of the manuscript.. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form.

Article Title: Stabilization and improved activity of arachidonate 11S-lipoxygenase from proteobacterium Myxococcus xanthus
Article Snippet: HFA standards, including 11S-HETE, 11R-HETE, 15S-HETE, 15R-HETE, 11-hydroxyeicosapentaenoic acid (HEPE), and 14-hydroxydocosahexaenoic acid (HDOHE), were purchased from Cayman Chemical.

Article Title: Peroxiredoxin III Protects Tumor Suppressor PTEN from Oxidation by 15-Hydroperoxy-eicosatetraenoic Acid
Article Snippet: 15s-HpETE and 15s-HETE were purchased from Cayman Chemical (Ann Arbor, MI, USA).

Article Title: Involvement of Proinflammatory Arachidonic Acid (ARA) Derivatives in Crohn’s Disease (CD) and Ulcerative Colitis (UC)
Article Snippet: Standards of ARA and LA derivatives: Thromboxane B2 (TXB2), Prostaglandin E2 (PGE2), Leukotriene B4 (LTB4), 13S-HODE (13S-hydroxy-9Z,11E-octadecadienoic acid), 9S-HODE (9S-hydroxy-10E, 12Z-octadecadienoic acid), 15S-HETE (15S-hydroxy-5Z,8Z,11Z,13E-eicosatetraenoic acid), 12S-HETE (12S-hydroxy-5Z,8Z,10E,14Z-eicosatetraenoic acid), 5-oxoETE (5-Oxo-eicosatetraenoic acid), 5-HETE (5-hydroxyeicosatetraenoic acid), 5(S),6(R)-lipoxin-A4 (5S,6R,15S-trihydroxy-7E,9E,11Z,13E-eicosatetraenoic acid), 5(S),6(R)15(R)-lipoxin-A4, (5(S),6(R),15(R)-trihydroxy 7E,9E,11Z,13E-eicosatetraenoic acid), 16RS-HETE Cayman Chemicals (Ann Arbor, MI, USA).

SDS Page:

Article Title: The crystal structure of Pseudomonas aeruginosa lipoxygenase Ala420Gly mutant explains the improved oxygen affinity and the altered reaction specificity.
Article Snippet: The crystal structure of Pseudomonas aeruginosa lipoxygenase Ala420Gly mutant explains the improved oxygen affinity and the altered reaction specificity Jacqueline Kalms, Swathi Banthiya, Etienne Galemou Yoga, Mats Hamberg, Hermann-Georg Holzhutter, Hartmut Kuhn, Patrick Scheerer PII: S1388-1981(17)30003-3 DOI: doi:10.1016/j.bbalip.2017.01.003 Reference: BBAMCB 58101 To appear in: BBA - Molecular and Cell Biology of Lipids Received date: 25 October 2016 Revised date: 3 January 2017 Accepted date: 12 January 2017 Please cite this article as: Jacqueline Kalms, Swathi Banthiya, Etienne Galemou Yoga, Mats Hamberg, Hermann-Georg Holzhutter, Hartmut Kuhn, Patrick Scheerer, The crystal structure of Pseudomonas aeruginosa lipoxygenase Ala420Gly mutant explains the improved oxygen affinity and the altered reaction specificity, BBA - Molecular and Cell Biology of Lipids (2017), doi:10.1016/j.bbalip.2017.01.003 This is a PDF file of an unedited manuscript that has been accepted for publication.. As a service to our customers we are providing this early version of the manuscript.. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form.

Article Title: Crystal Structure of Arachidonic Acid Bound to a Mutant of Prostaglandin Endoperoxide H Synthase-1 That Forms Predominantly 11-Hydroperoxyeicosatetraenoic Acid
Article Snippet: Materials—Arachidonic acid, 11R-HETE, and 15S-HETE were pur- chased from Cayman Chemical Co. (Ann Arbor, MI).

Article Title: Synergistic effect of 15-lipoxygenase 2 and radiation in killing head-and-neck cancer.
Article Snippet: 15S-HETE and AA were purchased from Cayman Chemical Inc. (An Arbor, MI).

Article Title: Strata movement and stress evolution when mining two overlapping panels affected by hard stratum
Article Snippet: 15s-HpETE and 15s-HETE were purchased from Cayman Chemical (Ann Arbor, MI, USA).

Article Title: Structural and functional basis of phospholipid oxygenase activity of bacterial lipoxygenase from Pseudomonas aeruginosa.
Article Snippet: Structural and functional basis of phospholipid oxygenase activity of bacterial lipoxygenase from Pseudomonas aeruginosa Swathi Banthiya, Jacqueline Kalms, Etienne Galemou Yoga, Igor Ivanov, Xavi Carpena, Mats Hamberg, Hartmut Kuhn, Patrick Scheerer PII: S1388-1981(16)30222-0 DOI: doi: 10.1016/j.bbalip.2016.08.002 Reference: BBAMCB 58023 To appear in: BBA - Molecular and Cell Biology of Lipids Received date: 19 May 2016 Revised date: 29 July 2016 Accepted date: 3 August 2016 Please cite this article as: Swathi Banthiya, Jacqueline Kalms, Etienne Galemou Yoga, Igor Ivanov, Xavi Carpena, Mats Hamberg, Hartmut Kuhn, Patrick Scheerer, Structural and functional basis of phospholipid oxygenase activity of bacterial lipoxygenase from Pseudomonas aeruginosa, BBA - Molecular and Cell Biology of Lipids (2016), doi: 10.1016/j.bbalip.2016.08.002 This is a PDF file of an unedited manuscript that has been accepted for publication.. As a service to our customers we are providing this early version of the manuscript.. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form.

Article Title: Stabilization and improved activity of arachidonate 11S-lipoxygenase from proteobacterium Myxococcus xanthus
Article Snippet: HFA standards, including 11S-HETE, 11R-HETE, 15S-HETE, 15R-HETE, 11-hydroxyeicosapentaenoic acid (HEPE), and 14-hydroxydocosahexaenoic acid (HDOHE), were purchased from Cayman Chemical.

Article Title: Peroxiredoxin III Protects Tumor Suppressor PTEN from Oxidation by 15-Hydroperoxy-eicosatetraenoic Acid
Article Snippet: 15s-HpETE and 15s-HETE were purchased from Cayman Chemical (Ann Arbor, MI, USA).

Article Title: Involvement of Proinflammatory Arachidonic Acid (ARA) Derivatives in Crohn’s Disease (CD) and Ulcerative Colitis (UC)
Article Snippet: Standards of ARA and LA derivatives: Thromboxane B2 (TXB2), Prostaglandin E2 (PGE2), Leukotriene B4 (LTB4), 13S-HODE (13S-hydroxy-9Z,11E-octadecadienoic acid), 9S-HODE (9S-hydroxy-10E, 12Z-octadecadienoic acid), 15S-HETE (15S-hydroxy-5Z,8Z,11Z,13E-eicosatetraenoic acid), 12S-HETE (12S-hydroxy-5Z,8Z,10E,14Z-eicosatetraenoic acid), 5-oxoETE (5-Oxo-eicosatetraenoic acid), 5-HETE (5-hydroxyeicosatetraenoic acid), 5(S),6(R)-lipoxin-A4 (5S,6R,15S-trihydroxy-7E,9E,11Z,13E-eicosatetraenoic acid), 5(S),6(R)15(R)-lipoxin-A4, (5(S),6(R),15(R)-trihydroxy 7E,9E,11Z,13E-eicosatetraenoic acid), 16RS-HETE Cayman Chemicals (Ann Arbor, MI, USA).

Western Blot:

Article Title: The crystal structure of Pseudomonas aeruginosa lipoxygenase Ala420Gly mutant explains the improved oxygen affinity and the altered reaction specificity.
Article Snippet: The crystal structure of Pseudomonas aeruginosa lipoxygenase Ala420Gly mutant explains the improved oxygen affinity and the altered reaction specificity Jacqueline Kalms, Swathi Banthiya, Etienne Galemou Yoga, Mats Hamberg, Hermann-Georg Holzhutter, Hartmut Kuhn, Patrick Scheerer PII: S1388-1981(17)30003-3 DOI: doi:10.1016/j.bbalip.2017.01.003 Reference: BBAMCB 58101 To appear in: BBA - Molecular and Cell Biology of Lipids Received date: 25 October 2016 Revised date: 3 January 2017 Accepted date: 12 January 2017 Please cite this article as: Jacqueline Kalms, Swathi Banthiya, Etienne Galemou Yoga, Mats Hamberg, Hermann-Georg Holzhutter, Hartmut Kuhn, Patrick Scheerer, The crystal structure of Pseudomonas aeruginosa lipoxygenase Ala420Gly mutant explains the improved oxygen affinity and the altered reaction specificity, BBA - Molecular and Cell Biology of Lipids (2017), doi:10.1016/j.bbalip.2017.01.003 This is a PDF file of an unedited manuscript that has been accepted for publication.. As a service to our customers we are providing this early version of the manuscript.. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form.

Article Title: Crystal Structure of Arachidonic Acid Bound to a Mutant of Prostaglandin Endoperoxide H Synthase-1 That Forms Predominantly 11-Hydroperoxyeicosatetraenoic Acid
Article Snippet: Materials—Arachidonic acid, 11R-HETE, and 15S-HETE were pur- chased from Cayman Chemical Co. (Ann Arbor, MI).

Article Title: Synergistic effect of 15-lipoxygenase 2 and radiation in killing head-and-neck cancer.
Article Snippet: 15S-HETE and AA were purchased from Cayman Chemical Inc. (An Arbor, MI).

Article Title: Strata movement and stress evolution when mining two overlapping panels affected by hard stratum
Article Snippet: 15s-HpETE and 15s-HETE were purchased from Cayman Chemical (Ann Arbor, MI, USA).

Article Title: Structural and functional basis of phospholipid oxygenase activity of bacterial lipoxygenase from Pseudomonas aeruginosa.
Article Snippet: Structural and functional basis of phospholipid oxygenase activity of bacterial lipoxygenase from Pseudomonas aeruginosa Swathi Banthiya, Jacqueline Kalms, Etienne Galemou Yoga, Igor Ivanov, Xavi Carpena, Mats Hamberg, Hartmut Kuhn, Patrick Scheerer PII: S1388-1981(16)30222-0 DOI: doi: 10.1016/j.bbalip.2016.08.002 Reference: BBAMCB 58023 To appear in: BBA - Molecular and Cell Biology of Lipids Received date: 19 May 2016 Revised date: 29 July 2016 Accepted date: 3 August 2016 Please cite this article as: Swathi Banthiya, Jacqueline Kalms, Etienne Galemou Yoga, Igor Ivanov, Xavi Carpena, Mats Hamberg, Hartmut Kuhn, Patrick Scheerer, Structural and functional basis of phospholipid oxygenase activity of bacterial lipoxygenase from Pseudomonas aeruginosa, BBA - Molecular and Cell Biology of Lipids (2016), doi: 10.1016/j.bbalip.2016.08.002 This is a PDF file of an unedited manuscript that has been accepted for publication.. As a service to our customers we are providing this early version of the manuscript.. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form.

Article Title: Stabilization and improved activity of arachidonate 11S-lipoxygenase from proteobacterium Myxococcus xanthus
Article Snippet: HFA standards, including 11S-HETE, 11R-HETE, 15S-HETE, 15R-HETE, 11-hydroxyeicosapentaenoic acid (HEPE), and 14-hydroxydocosahexaenoic acid (HDOHE), were purchased from Cayman Chemical.

Article Title: Peroxiredoxin III Protects Tumor Suppressor PTEN from Oxidation by 15-Hydroperoxy-eicosatetraenoic Acid
Article Snippet: 15s-HpETE and 15s-HETE were purchased from Cayman Chemical (Ann Arbor, MI, USA).

Article Title: Involvement of Proinflammatory Arachidonic Acid (ARA) Derivatives in Crohn’s Disease (CD) and Ulcerative Colitis (UC)
Article Snippet: Standards of ARA and LA derivatives: Thromboxane B2 (TXB2), Prostaglandin E2 (PGE2), Leukotriene B4 (LTB4), 13S-HODE (13S-hydroxy-9Z,11E-octadecadienoic acid), 9S-HODE (9S-hydroxy-10E, 12Z-octadecadienoic acid), 15S-HETE (15S-hydroxy-5Z,8Z,11Z,13E-eicosatetraenoic acid), 12S-HETE (12S-hydroxy-5Z,8Z,10E,14Z-eicosatetraenoic acid), 5-oxoETE (5-Oxo-eicosatetraenoic acid), 5-HETE (5-hydroxyeicosatetraenoic acid), 5(S),6(R)-lipoxin-A4 (5S,6R,15S-trihydroxy-7E,9E,11Z,13E-eicosatetraenoic acid), 5(S),6(R)15(R)-lipoxin-A4, (5(S),6(R),15(R)-trihydroxy 7E,9E,11Z,13E-eicosatetraenoic acid), 16RS-HETE Cayman Chemicals (Ann Arbor, MI, USA).

Acetylene Reduction Assay:

Article Title: The crystal structure of Pseudomonas aeruginosa lipoxygenase Ala420Gly mutant explains the improved oxygen affinity and the altered reaction specificity.
Article Snippet: The crystal structure of Pseudomonas aeruginosa lipoxygenase Ala420Gly mutant explains the improved oxygen affinity and the altered reaction specificity Jacqueline Kalms, Swathi Banthiya, Etienne Galemou Yoga, Mats Hamberg, Hermann-Georg Holzhutter, Hartmut Kuhn, Patrick Scheerer PII: S1388-1981(17)30003-3 DOI: doi:10.1016/j.bbalip.2017.01.003 Reference: BBAMCB 58101 To appear in: BBA - Molecular and Cell Biology of Lipids Received date: 25 October 2016 Revised date: 3 January 2017 Accepted date: 12 January 2017 Please cite this article as: Jacqueline Kalms, Swathi Banthiya, Etienne Galemou Yoga, Mats Hamberg, Hermann-Georg Holzhutter, Hartmut Kuhn, Patrick Scheerer, The crystal structure of Pseudomonas aeruginosa lipoxygenase Ala420Gly mutant explains the improved oxygen affinity and the altered reaction specificity, BBA - Molecular and Cell Biology of Lipids (2017), doi:10.1016/j.bbalip.2017.01.003 This is a PDF file of an unedited manuscript that has been accepted for publication.. As a service to our customers we are providing this early version of the manuscript.. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form.

Article Title: Crystal Structure of Arachidonic Acid Bound to a Mutant of Prostaglandin Endoperoxide H Synthase-1 That Forms Predominantly 11-Hydroperoxyeicosatetraenoic Acid
Article Snippet: Materials—Arachidonic acid, 11R-HETE, and 15S-HETE were pur- chased from Cayman Chemical Co. (Ann Arbor, MI).

Article Title: Synergistic effect of 15-lipoxygenase 2 and radiation in killing head-and-neck cancer.
Article Snippet: 15S-HETE and AA were purchased from Cayman Chemical Inc. (An Arbor, MI).

Article Title: Strata movement and stress evolution when mining two overlapping panels affected by hard stratum
Article Snippet: 15s-HpETE and 15s-HETE were purchased from Cayman Chemical (Ann Arbor, MI, USA).

Article Title: Structural and functional basis of phospholipid oxygenase activity of bacterial lipoxygenase from Pseudomonas aeruginosa.
Article Snippet: Structural and functional basis of phospholipid oxygenase activity of bacterial lipoxygenase from Pseudomonas aeruginosa Swathi Banthiya, Jacqueline Kalms, Etienne Galemou Yoga, Igor Ivanov, Xavi Carpena, Mats Hamberg, Hartmut Kuhn, Patrick Scheerer PII: S1388-1981(16)30222-0 DOI: doi: 10.1016/j.bbalip.2016.08.002 Reference: BBAMCB 58023 To appear in: BBA - Molecular and Cell Biology of Lipids Received date: 19 May 2016 Revised date: 29 July 2016 Accepted date: 3 August 2016 Please cite this article as: Swathi Banthiya, Jacqueline Kalms, Etienne Galemou Yoga, Igor Ivanov, Xavi Carpena, Mats Hamberg, Hartmut Kuhn, Patrick Scheerer, Structural and functional basis of phospholipid oxygenase activity of bacterial lipoxygenase from Pseudomonas aeruginosa, BBA - Molecular and Cell Biology of Lipids (2016), doi: 10.1016/j.bbalip.2016.08.002 This is a PDF file of an unedited manuscript that has been accepted for publication.. As a service to our customers we are providing this early version of the manuscript.. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form.

Article Title: Stabilization and improved activity of arachidonate 11S-lipoxygenase from proteobacterium Myxococcus xanthus
Article Snippet: HFA standards, including 11S-HETE, 11R-HETE, 15S-HETE, 15R-HETE, 11-hydroxyeicosapentaenoic acid (HEPE), and 14-hydroxydocosahexaenoic acid (HDOHE), were purchased from Cayman Chemical.

Article Title: Peroxiredoxin III Protects Tumor Suppressor PTEN from Oxidation by 15-Hydroperoxy-eicosatetraenoic Acid
Article Snippet: 15s-HpETE and 15s-HETE were purchased from Cayman Chemical (Ann Arbor, MI, USA).

Article Title: Involvement of Proinflammatory Arachidonic Acid (ARA) Derivatives in Crohn’s Disease (CD) and Ulcerative Colitis (UC)
Article Snippet: Standards of ARA and LA derivatives: Thromboxane B2 (TXB2), Prostaglandin E2 (PGE2), Leukotriene B4 (LTB4), 13S-HODE (13S-hydroxy-9Z,11E-octadecadienoic acid), 9S-HODE (9S-hydroxy-10E, 12Z-octadecadienoic acid), 15S-HETE (15S-hydroxy-5Z,8Z,11Z,13E-eicosatetraenoic acid), 12S-HETE (12S-hydroxy-5Z,8Z,10E,14Z-eicosatetraenoic acid), 5-oxoETE (5-Oxo-eicosatetraenoic acid), 5-HETE (5-hydroxyeicosatetraenoic acid), 5(S),6(R)-lipoxin-A4 (5S,6R,15S-trihydroxy-7E,9E,11Z,13E-eicosatetraenoic acid), 5(S),6(R)15(R)-lipoxin-A4, (5(S),6(R),15(R)-trihydroxy 7E,9E,11Z,13E-eicosatetraenoic acid), 16RS-HETE Cayman Chemicals (Ann Arbor, MI, USA).

Control:

Article Title: The crystal structure of Pseudomonas aeruginosa lipoxygenase Ala420Gly mutant explains the improved oxygen affinity and the altered reaction specificity.
Article Snippet: The crystal structure of Pseudomonas aeruginosa lipoxygenase Ala420Gly mutant explains the improved oxygen affinity and the altered reaction specificity Jacqueline Kalms, Swathi Banthiya, Etienne Galemou Yoga, Mats Hamberg, Hermann-Georg Holzhutter, Hartmut Kuhn, Patrick Scheerer PII: S1388-1981(17)30003-3 DOI: doi:10.1016/j.bbalip.2017.01.003 Reference: BBAMCB 58101 To appear in: BBA - Molecular and Cell Biology of Lipids Received date: 25 October 2016 Revised date: 3 January 2017 Accepted date: 12 January 2017 Please cite this article as: Jacqueline Kalms, Swathi Banthiya, Etienne Galemou Yoga, Mats Hamberg, Hermann-Georg Holzhutter, Hartmut Kuhn, Patrick Scheerer, The crystal structure of Pseudomonas aeruginosa lipoxygenase Ala420Gly mutant explains the improved oxygen affinity and the altered reaction specificity, BBA - Molecular and Cell Biology of Lipids (2017), doi:10.1016/j.bbalip.2017.01.003 This is a PDF file of an unedited manuscript that has been accepted for publication.. As a service to our customers we are providing this early version of the manuscript.. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form.

Article Title: Crystal Structure of Arachidonic Acid Bound to a Mutant of Prostaglandin Endoperoxide H Synthase-1 That Forms Predominantly 11-Hydroperoxyeicosatetraenoic Acid
Article Snippet: Materials—Arachidonic acid, 11R-HETE, and 15S-HETE were pur- chased from Cayman Chemical Co. (Ann Arbor, MI).

Article Title: Synergistic effect of 15-lipoxygenase 2 and radiation in killing head-and-neck cancer.
Article Snippet: 15S-HETE and AA were purchased from Cayman Chemical Inc. (An Arbor, MI).

Article Title: Strata movement and stress evolution when mining two overlapping panels affected by hard stratum
Article Snippet: 15s-HpETE and 15s-HETE were purchased from Cayman Chemical (Ann Arbor, MI, USA).

Article Title: Structural and functional basis of phospholipid oxygenase activity of bacterial lipoxygenase from Pseudomonas aeruginosa.
Article Snippet: Structural and functional basis of phospholipid oxygenase activity of bacterial lipoxygenase from Pseudomonas aeruginosa Swathi Banthiya, Jacqueline Kalms, Etienne Galemou Yoga, Igor Ivanov, Xavi Carpena, Mats Hamberg, Hartmut Kuhn, Patrick Scheerer PII: S1388-1981(16)30222-0 DOI: doi: 10.1016/j.bbalip.2016.08.002 Reference: BBAMCB 58023 To appear in: BBA - Molecular and Cell Biology of Lipids Received date: 19 May 2016 Revised date: 29 July 2016 Accepted date: 3 August 2016 Please cite this article as: Swathi Banthiya, Jacqueline Kalms, Etienne Galemou Yoga, Igor Ivanov, Xavi Carpena, Mats Hamberg, Hartmut Kuhn, Patrick Scheerer, Structural and functional basis of phospholipid oxygenase activity of bacterial lipoxygenase from Pseudomonas aeruginosa, BBA - Molecular and Cell Biology of Lipids (2016), doi: 10.1016/j.bbalip.2016.08.002 This is a PDF file of an unedited manuscript that has been accepted for publication.. As a service to our customers we are providing this early version of the manuscript.. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form.

Article Title: Stabilization and improved activity of arachidonate 11S-lipoxygenase from proteobacterium Myxococcus xanthus
Article Snippet: HFA standards, including 11S-HETE, 11R-HETE, 15S-HETE, 15R-HETE, 11-hydroxyeicosapentaenoic acid (HEPE), and 14-hydroxydocosahexaenoic acid (HDOHE), were purchased from Cayman Chemical.

Article Title: Peroxiredoxin III Protects Tumor Suppressor PTEN from Oxidation by 15-Hydroperoxy-eicosatetraenoic Acid
Article Snippet: 15s-HpETE and 15s-HETE were purchased from Cayman Chemical (Ann Arbor, MI, USA).

Article Title: Involvement of Proinflammatory Arachidonic Acid (ARA) Derivatives in Crohn’s Disease (CD) and Ulcerative Colitis (UC)
Article Snippet: Standards of ARA and LA derivatives: Thromboxane B2 (TXB2), Prostaglandin E2 (PGE2), Leukotriene B4 (LTB4), 13S-HODE (13S-hydroxy-9Z,11E-octadecadienoic acid), 9S-HODE (9S-hydroxy-10E, 12Z-octadecadienoic acid), 15S-HETE (15S-hydroxy-5Z,8Z,11Z,13E-eicosatetraenoic acid), 12S-HETE (12S-hydroxy-5Z,8Z,10E,14Z-eicosatetraenoic acid), 5-oxoETE (5-Oxo-eicosatetraenoic acid), 5-HETE (5-hydroxyeicosatetraenoic acid), 5(S),6(R)-lipoxin-A4 (5S,6R,15S-trihydroxy-7E,9E,11Z,13E-eicosatetraenoic acid), 5(S),6(R)15(R)-lipoxin-A4, (5(S),6(R),15(R)-trihydroxy 7E,9E,11Z,13E-eicosatetraenoic acid), 16RS-HETE Cayman Chemicals (Ann Arbor, MI, USA).

Activity Assay:

Article Title: The crystal structure of Pseudomonas aeruginosa lipoxygenase Ala420Gly mutant explains the improved oxygen affinity and the altered reaction specificity.
Article Snippet: The crystal structure of Pseudomonas aeruginosa lipoxygenase Ala420Gly mutant explains the improved oxygen affinity and the altered reaction specificity Jacqueline Kalms, Swathi Banthiya, Etienne Galemou Yoga, Mats Hamberg, Hermann-Georg Holzhutter, Hartmut Kuhn, Patrick Scheerer PII: S1388-1981(17)30003-3 DOI: doi:10.1016/j.bbalip.2017.01.003 Reference: BBAMCB 58101 To appear in: BBA - Molecular and Cell Biology of Lipids Received date: 25 October 2016 Revised date: 3 January 2017 Accepted date: 12 January 2017 Please cite this article as: Jacqueline Kalms, Swathi Banthiya, Etienne Galemou Yoga, Mats Hamberg, Hermann-Georg Holzhutter, Hartmut Kuhn, Patrick Scheerer, The crystal structure of Pseudomonas aeruginosa lipoxygenase Ala420Gly mutant explains the improved oxygen affinity and the altered reaction specificity, BBA - Molecular and Cell Biology of Lipids (2017), doi:10.1016/j.bbalip.2017.01.003 This is a PDF file of an unedited manuscript that has been accepted for publication.. As a service to our customers we are providing this early version of the manuscript.. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form.

Article Title: Crystal Structure of Arachidonic Acid Bound to a Mutant of Prostaglandin Endoperoxide H Synthase-1 That Forms Predominantly 11-Hydroperoxyeicosatetraenoic Acid
Article Snippet: Materials—Arachidonic acid, 11R-HETE, and 15S-HETE were pur- chased from Cayman Chemical Co. (Ann Arbor, MI).

Article Title: Synergistic effect of 15-lipoxygenase 2 and radiation in killing head-and-neck cancer.
Article Snippet: 15S-HETE and AA were purchased from Cayman Chemical Inc. (An Arbor, MI).

Article Title: Strata movement and stress evolution when mining two overlapping panels affected by hard stratum
Article Snippet: 15s-HpETE and 15s-HETE were purchased from Cayman Chemical (Ann Arbor, MI, USA).

Article Title: Structural and functional basis of phospholipid oxygenase activity of bacterial lipoxygenase from Pseudomonas aeruginosa.
Article Snippet: Structural and functional basis of phospholipid oxygenase activity of bacterial lipoxygenase from Pseudomonas aeruginosa Swathi Banthiya, Jacqueline Kalms, Etienne Galemou Yoga, Igor Ivanov, Xavi Carpena, Mats Hamberg, Hartmut Kuhn, Patrick Scheerer PII: S1388-1981(16)30222-0 DOI: doi: 10.1016/j.bbalip.2016.08.002 Reference: BBAMCB 58023 To appear in: BBA - Molecular and Cell Biology of Lipids Received date: 19 May 2016 Revised date: 29 July 2016 Accepted date: 3 August 2016 Please cite this article as: Swathi Banthiya, Jacqueline Kalms, Etienne Galemou Yoga, Igor Ivanov, Xavi Carpena, Mats Hamberg, Hartmut Kuhn, Patrick Scheerer, Structural and functional basis of phospholipid oxygenase activity of bacterial lipoxygenase from Pseudomonas aeruginosa, BBA - Molecular and Cell Biology of Lipids (2016), doi: 10.1016/j.bbalip.2016.08.002 This is a PDF file of an unedited manuscript that has been accepted for publication.. As a service to our customers we are providing this early version of the manuscript.. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form.

Article Title: Stabilization and improved activity of arachidonate 11S-lipoxygenase from proteobacterium Myxococcus xanthus
Article Snippet: HFA standards, including 11S-HETE, 11R-HETE, 15S-HETE, 15R-HETE, 11-hydroxyeicosapentaenoic acid (HEPE), and 14-hydroxydocosahexaenoic acid (HDOHE), were purchased from Cayman Chemical.

Article Title: Peroxiredoxin III Protects Tumor Suppressor PTEN from Oxidation by 15-Hydroperoxy-eicosatetraenoic Acid
Article Snippet: 15s-HpETE and 15s-HETE were purchased from Cayman Chemical (Ann Arbor, MI, USA).

Article Title: Involvement of Proinflammatory Arachidonic Acid (ARA) Derivatives in Crohn’s Disease (CD) and Ulcerative Colitis (UC)
Article Snippet: Standards of ARA and LA derivatives: Thromboxane B2 (TXB2), Prostaglandin E2 (PGE2), Leukotriene B4 (LTB4), 13S-HODE (13S-hydroxy-9Z,11E-octadecadienoic acid), 9S-HODE (9S-hydroxy-10E, 12Z-octadecadienoic acid), 15S-HETE (15S-hydroxy-5Z,8Z,11Z,13E-eicosatetraenoic acid), 12S-HETE (12S-hydroxy-5Z,8Z,10E,14Z-eicosatetraenoic acid), 5-oxoETE (5-Oxo-eicosatetraenoic acid), 5-HETE (5-hydroxyeicosatetraenoic acid), 5(S),6(R)-lipoxin-A4 (5S,6R,15S-trihydroxy-7E,9E,11Z,13E-eicosatetraenoic acid), 5(S),6(R)15(R)-lipoxin-A4, (5(S),6(R),15(R)-trihydroxy 7E,9E,11Z,13E-eicosatetraenoic acid), 16RS-HETE Cayman Chemicals (Ann Arbor, MI, USA).

Concentration Assay:

Article Title: The crystal structure of Pseudomonas aeruginosa lipoxygenase Ala420Gly mutant explains the improved oxygen affinity and the altered reaction specificity.
Article Snippet: The crystal structure of Pseudomonas aeruginosa lipoxygenase Ala420Gly mutant explains the improved oxygen affinity and the altered reaction specificity Jacqueline Kalms, Swathi Banthiya, Etienne Galemou Yoga, Mats Hamberg, Hermann-Georg Holzhutter, Hartmut Kuhn, Patrick Scheerer PII: S1388-1981(17)30003-3 DOI: doi:10.1016/j.bbalip.2017.01.003 Reference: BBAMCB 58101 To appear in: BBA - Molecular and Cell Biology of Lipids Received date: 25 October 2016 Revised date: 3 January 2017 Accepted date: 12 January 2017 Please cite this article as: Jacqueline Kalms, Swathi Banthiya, Etienne Galemou Yoga, Mats Hamberg, Hermann-Georg Holzhutter, Hartmut Kuhn, Patrick Scheerer, The crystal structure of Pseudomonas aeruginosa lipoxygenase Ala420Gly mutant explains the improved oxygen affinity and the altered reaction specificity, BBA - Molecular and Cell Biology of Lipids (2017), doi:10.1016/j.bbalip.2017.01.003 This is a PDF file of an unedited manuscript that has been accepted for publication.. As a service to our customers we are providing this early version of the manuscript.. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form.

Article Title: Crystal Structure of Arachidonic Acid Bound to a Mutant of Prostaglandin Endoperoxide H Synthase-1 That Forms Predominantly 11-Hydroperoxyeicosatetraenoic Acid
Article Snippet: Materials—Arachidonic acid, 11R-HETE, and 15S-HETE were pur- chased from Cayman Chemical Co. (Ann Arbor, MI).

Article Title: Synergistic effect of 15-lipoxygenase 2 and radiation in killing head-and-neck cancer.
Article Snippet: 15S-HETE and AA were purchased from Cayman Chemical Inc. (An Arbor, MI).

Article Title: Strata movement and stress evolution when mining two overlapping panels affected by hard stratum
Article Snippet: 15s-HpETE and 15s-HETE were purchased from Cayman Chemical (Ann Arbor, MI, USA).

Article Title: Structural and functional basis of phospholipid oxygenase activity of bacterial lipoxygenase from Pseudomonas aeruginosa.
Article Snippet: Structural and functional basis of phospholipid oxygenase activity of bacterial lipoxygenase from Pseudomonas aeruginosa Swathi Banthiya, Jacqueline Kalms, Etienne Galemou Yoga, Igor Ivanov, Xavi Carpena, Mats Hamberg, Hartmut Kuhn, Patrick Scheerer PII: S1388-1981(16)30222-0 DOI: doi: 10.1016/j.bbalip.2016.08.002 Reference: BBAMCB 58023 To appear in: BBA - Molecular and Cell Biology of Lipids Received date: 19 May 2016 Revised date: 29 July 2016 Accepted date: 3 August 2016 Please cite this article as: Swathi Banthiya, Jacqueline Kalms, Etienne Galemou Yoga, Igor Ivanov, Xavi Carpena, Mats Hamberg, Hartmut Kuhn, Patrick Scheerer, Structural and functional basis of phospholipid oxygenase activity of bacterial lipoxygenase from Pseudomonas aeruginosa, BBA - Molecular and Cell Biology of Lipids (2016), doi: 10.1016/j.bbalip.2016.08.002 This is a PDF file of an unedited manuscript that has been accepted for publication.. As a service to our customers we are providing this early version of the manuscript.. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form.

Article Title: Stabilization and improved activity of arachidonate 11S-lipoxygenase from proteobacterium Myxococcus xanthus
Article Snippet: HFA standards, including 11S-HETE, 11R-HETE, 15S-HETE, 15R-HETE, 11-hydroxyeicosapentaenoic acid (HEPE), and 14-hydroxydocosahexaenoic acid (HDOHE), were purchased from Cayman Chemical.

Article Title: Peroxiredoxin III Protects Tumor Suppressor PTEN from Oxidation by 15-Hydroperoxy-eicosatetraenoic Acid
Article Snippet: 15s-HpETE and 15s-HETE were purchased from Cayman Chemical (Ann Arbor, MI, USA).

Article Title: Involvement of Proinflammatory Arachidonic Acid (ARA) Derivatives in Crohn’s Disease (CD) and Ulcerative Colitis (UC)
Article Snippet: Standards of ARA and LA derivatives: Thromboxane B2 (TXB2), Prostaglandin E2 (PGE2), Leukotriene B4 (LTB4), 13S-HODE (13S-hydroxy-9Z,11E-octadecadienoic acid), 9S-HODE (9S-hydroxy-10E, 12Z-octadecadienoic acid), 15S-HETE (15S-hydroxy-5Z,8Z,11Z,13E-eicosatetraenoic acid), 12S-HETE (12S-hydroxy-5Z,8Z,10E,14Z-eicosatetraenoic acid), 5-oxoETE (5-Oxo-eicosatetraenoic acid), 5-HETE (5-hydroxyeicosatetraenoic acid), 5(S),6(R)-lipoxin-A4 (5S,6R,15S-trihydroxy-7E,9E,11Z,13E-eicosatetraenoic acid), 5(S),6(R)15(R)-lipoxin-A4, (5(S),6(R),15(R)-trihydroxy 7E,9E,11Z,13E-eicosatetraenoic acid), 16RS-HETE Cayman Chemicals (Ann Arbor, MI, USA).

Expressing:

Article Title: The crystal structure of Pseudomonas aeruginosa lipoxygenase Ala420Gly mutant explains the improved oxygen affinity and the altered reaction specificity.
Article Snippet: The crystal structure of Pseudomonas aeruginosa lipoxygenase Ala420Gly mutant explains the improved oxygen affinity and the altered reaction specificity Jacqueline Kalms, Swathi Banthiya, Etienne Galemou Yoga, Mats Hamberg, Hermann-Georg Holzhutter, Hartmut Kuhn, Patrick Scheerer PII: S1388-1981(17)30003-3 DOI: doi:10.1016/j.bbalip.2017.01.003 Reference: BBAMCB 58101 To appear in: BBA - Molecular and Cell Biology of Lipids Received date: 25 October 2016 Revised date: 3 January 2017 Accepted date: 12 January 2017 Please cite this article as: Jacqueline Kalms, Swathi Banthiya, Etienne Galemou Yoga, Mats Hamberg, Hermann-Georg Holzhutter, Hartmut Kuhn, Patrick Scheerer, The crystal structure of Pseudomonas aeruginosa lipoxygenase Ala420Gly mutant explains the improved oxygen affinity and the altered reaction specificity, BBA - Molecular and Cell Biology of Lipids (2017), doi:10.1016/j.bbalip.2017.01.003 This is a PDF file of an unedited manuscript that has been accepted for publication.. As a service to our customers we are providing this early version of the manuscript.. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form.

Article Title: Crystal Structure of Arachidonic Acid Bound to a Mutant of Prostaglandin Endoperoxide H Synthase-1 That Forms Predominantly 11-Hydroperoxyeicosatetraenoic Acid
Article Snippet: Materials—Arachidonic acid, 11R-HETE, and 15S-HETE were pur- chased from Cayman Chemical Co. (Ann Arbor, MI).

Article Title: Synergistic effect of 15-lipoxygenase 2 and radiation in killing head-and-neck cancer.
Article Snippet: 15S-HETE and AA were purchased from Cayman Chemical Inc. (An Arbor, MI).

Article Title: Strata movement and stress evolution when mining two overlapping panels affected by hard stratum
Article Snippet: 15s-HpETE and 15s-HETE were purchased from Cayman Chemical (Ann Arbor, MI, USA).

Article Title: Structural and functional basis of phospholipid oxygenase activity of bacterial lipoxygenase from Pseudomonas aeruginosa.
Article Snippet: Structural and functional basis of phospholipid oxygenase activity of bacterial lipoxygenase from Pseudomonas aeruginosa Swathi Banthiya, Jacqueline Kalms, Etienne Galemou Yoga, Igor Ivanov, Xavi Carpena, Mats Hamberg, Hartmut Kuhn, Patrick Scheerer PII: S1388-1981(16)30222-0 DOI: doi: 10.1016/j.bbalip.2016.08.002 Reference: BBAMCB 58023 To appear in: BBA - Molecular and Cell Biology of Lipids Received date: 19 May 2016 Revised date: 29 July 2016 Accepted date: 3 August 2016 Please cite this article as: Swathi Banthiya, Jacqueline Kalms, Etienne Galemou Yoga, Igor Ivanov, Xavi Carpena, Mats Hamberg, Hartmut Kuhn, Patrick Scheerer, Structural and functional basis of phospholipid oxygenase activity of bacterial lipoxygenase from Pseudomonas aeruginosa, BBA - Molecular and Cell Biology of Lipids (2016), doi: 10.1016/j.bbalip.2016.08.002 This is a PDF file of an unedited manuscript that has been accepted for publication.. As a service to our customers we are providing this early version of the manuscript.. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form.

Article Title: Stabilization and improved activity of arachidonate 11S-lipoxygenase from proteobacterium Myxococcus xanthus
Article Snippet: HFA standards, including 11S-HETE, 11R-HETE, 15S-HETE, 15R-HETE, 11-hydroxyeicosapentaenoic acid (HEPE), and 14-hydroxydocosahexaenoic acid (HDOHE), were purchased from Cayman Chemical.

Article Title: Peroxiredoxin III Protects Tumor Suppressor PTEN from Oxidation by 15-Hydroperoxy-eicosatetraenoic Acid
Article Snippet: 15s-HpETE and 15s-HETE were purchased from Cayman Chemical (Ann Arbor, MI, USA).

Article Title: Involvement of Proinflammatory Arachidonic Acid (ARA) Derivatives in Crohn’s Disease (CD) and Ulcerative Colitis (UC)
Article Snippet: Standards of ARA and LA derivatives: Thromboxane B2 (TXB2), Prostaglandin E2 (PGE2), Leukotriene B4 (LTB4), 13S-HODE (13S-hydroxy-9Z,11E-octadecadienoic acid), 9S-HODE (9S-hydroxy-10E, 12Z-octadecadienoic acid), 15S-HETE (15S-hydroxy-5Z,8Z,11Z,13E-eicosatetraenoic acid), 12S-HETE (12S-hydroxy-5Z,8Z,10E,14Z-eicosatetraenoic acid), 5-oxoETE (5-Oxo-eicosatetraenoic acid), 5-HETE (5-hydroxyeicosatetraenoic acid), 5(S),6(R)-lipoxin-A4 (5S,6R,15S-trihydroxy-7E,9E,11Z,13E-eicosatetraenoic acid), 5(S),6(R)15(R)-lipoxin-A4, (5(S),6(R),15(R)-trihydroxy 7E,9E,11Z,13E-eicosatetraenoic acid), 16RS-HETE Cayman Chemicals (Ann Arbor, MI, USA).

Clinical Proteomics:

Article Title: The crystal structure of Pseudomonas aeruginosa lipoxygenase Ala420Gly mutant explains the improved oxygen affinity and the altered reaction specificity.
Article Snippet: The crystal structure of Pseudomonas aeruginosa lipoxygenase Ala420Gly mutant explains the improved oxygen affinity and the altered reaction specificity Jacqueline Kalms, Swathi Banthiya, Etienne Galemou Yoga, Mats Hamberg, Hermann-Georg Holzhutter, Hartmut Kuhn, Patrick Scheerer PII: S1388-1981(17)30003-3 DOI: doi:10.1016/j.bbalip.2017.01.003 Reference: BBAMCB 58101 To appear in: BBA - Molecular and Cell Biology of Lipids Received date: 25 October 2016 Revised date: 3 January 2017 Accepted date: 12 January 2017 Please cite this article as: Jacqueline Kalms, Swathi Banthiya, Etienne Galemou Yoga, Mats Hamberg, Hermann-Georg Holzhutter, Hartmut Kuhn, Patrick Scheerer, The crystal structure of Pseudomonas aeruginosa lipoxygenase Ala420Gly mutant explains the improved oxygen affinity and the altered reaction specificity, BBA - Molecular and Cell Biology of Lipids (2017), doi:10.1016/j.bbalip.2017.01.003 This is a PDF file of an unedited manuscript that has been accepted for publication.. As a service to our customers we are providing this early version of the manuscript.. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form.

Article Title: Crystal Structure of Arachidonic Acid Bound to a Mutant of Prostaglandin Endoperoxide H Synthase-1 That Forms Predominantly 11-Hydroperoxyeicosatetraenoic Acid
Article Snippet: Materials—Arachidonic acid, 11R-HETE, and 15S-HETE were pur- chased from Cayman Chemical Co. (Ann Arbor, MI).

Article Title: Synergistic effect of 15-lipoxygenase 2 and radiation in killing head-and-neck cancer.
Article Snippet: 15S-HETE and AA were purchased from Cayman Chemical Inc. (An Arbor, MI).

Article Title: Strata movement and stress evolution when mining two overlapping panels affected by hard stratum
Article Snippet: 15s-HpETE and 15s-HETE were purchased from Cayman Chemical (Ann Arbor, MI, USA).

Article Title: Structural and functional basis of phospholipid oxygenase activity of bacterial lipoxygenase from Pseudomonas aeruginosa.
Article Snippet: Structural and functional basis of phospholipid oxygenase activity of bacterial lipoxygenase from Pseudomonas aeruginosa Swathi Banthiya, Jacqueline Kalms, Etienne Galemou Yoga, Igor Ivanov, Xavi Carpena, Mats Hamberg, Hartmut Kuhn, Patrick Scheerer PII: S1388-1981(16)30222-0 DOI: doi: 10.1016/j.bbalip.2016.08.002 Reference: BBAMCB 58023 To appear in: BBA - Molecular and Cell Biology of Lipids Received date: 19 May 2016 Revised date: 29 July 2016 Accepted date: 3 August 2016 Please cite this article as: Swathi Banthiya, Jacqueline Kalms, Etienne Galemou Yoga, Igor Ivanov, Xavi Carpena, Mats Hamberg, Hartmut Kuhn, Patrick Scheerer, Structural and functional basis of phospholipid oxygenase activity of bacterial lipoxygenase from Pseudomonas aeruginosa, BBA - Molecular and Cell Biology of Lipids (2016), doi: 10.1016/j.bbalip.2016.08.002 This is a PDF file of an unedited manuscript that has been accepted for publication.. As a service to our customers we are providing this early version of the manuscript.. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form.

Article Title: Stabilization and improved activity of arachidonate 11S-lipoxygenase from proteobacterium Myxococcus xanthus
Article Snippet: HFA standards, including 11S-HETE, 11R-HETE, 15S-HETE, 15R-HETE, 11-hydroxyeicosapentaenoic acid (HEPE), and 14-hydroxydocosahexaenoic acid (HDOHE), were purchased from Cayman Chemical.

Article Title: Peroxiredoxin III Protects Tumor Suppressor PTEN from Oxidation by 15-Hydroperoxy-eicosatetraenoic Acid
Article Snippet: 15s-HpETE and 15s-HETE were purchased from Cayman Chemical (Ann Arbor, MI, USA).

Article Title: Involvement of Proinflammatory Arachidonic Acid (ARA) Derivatives in Crohn’s Disease (CD) and Ulcerative Colitis (UC)
Article Snippet: Standards of ARA and LA derivatives: Thromboxane B2 (TXB2), Prostaglandin E2 (PGE2), Leukotriene B4 (LTB4), 13S-HODE (13S-hydroxy-9Z,11E-octadecadienoic acid), 9S-HODE (9S-hydroxy-10E, 12Z-octadecadienoic acid), 15S-HETE (15S-hydroxy-5Z,8Z,11Z,13E-eicosatetraenoic acid), 12S-HETE (12S-hydroxy-5Z,8Z,10E,14Z-eicosatetraenoic acid), 5-oxoETE (5-Oxo-eicosatetraenoic acid), 5-HETE (5-hydroxyeicosatetraenoic acid), 5(S),6(R)-lipoxin-A4 (5S,6R,15S-trihydroxy-7E,9E,11Z,13E-eicosatetraenoic acid), 5(S),6(R)15(R)-lipoxin-A4, (5(S),6(R),15(R)-trihydroxy 7E,9E,11Z,13E-eicosatetraenoic acid), 16RS-HETE Cayman Chemicals (Ann Arbor, MI, USA).

Drug discovery:

Article Title: The crystal structure of Pseudomonas aeruginosa lipoxygenase Ala420Gly mutant explains the improved oxygen affinity and the altered reaction specificity.
Article Snippet: The crystal structure of Pseudomonas aeruginosa lipoxygenase Ala420Gly mutant explains the improved oxygen affinity and the altered reaction specificity Jacqueline Kalms, Swathi Banthiya, Etienne Galemou Yoga, Mats Hamberg, Hermann-Georg Holzhutter, Hartmut Kuhn, Patrick Scheerer PII: S1388-1981(17)30003-3 DOI: doi:10.1016/j.bbalip.2017.01.003 Reference: BBAMCB 58101 To appear in: BBA - Molecular and Cell Biology of Lipids Received date: 25 October 2016 Revised date: 3 January 2017 Accepted date: 12 January 2017 Please cite this article as: Jacqueline Kalms, Swathi Banthiya, Etienne Galemou Yoga, Mats Hamberg, Hermann-Georg Holzhutter, Hartmut Kuhn, Patrick Scheerer, The crystal structure of Pseudomonas aeruginosa lipoxygenase Ala420Gly mutant explains the improved oxygen affinity and the altered reaction specificity, BBA - Molecular and Cell Biology of Lipids (2017), doi:10.1016/j.bbalip.2017.01.003 This is a PDF file of an unedited manuscript that has been accepted for publication.. As a service to our customers we are providing this early version of the manuscript.. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form.

Article Title: Crystal Structure of Arachidonic Acid Bound to a Mutant of Prostaglandin Endoperoxide H Synthase-1 That Forms Predominantly 11-Hydroperoxyeicosatetraenoic Acid
Article Snippet: Materials—Arachidonic acid, 11R-HETE, and 15S-HETE were pur- chased from Cayman Chemical Co. (Ann Arbor, MI).

Article Title: Synergistic effect of 15-lipoxygenase 2 and radiation in killing head-and-neck cancer.
Article Snippet: 15S-HETE and AA were purchased from Cayman Chemical Inc. (An Arbor, MI).

Article Title: Strata movement and stress evolution when mining two overlapping panels affected by hard stratum
Article Snippet: 15s-HpETE and 15s-HETE were purchased from Cayman Chemical (Ann Arbor, MI, USA).

Article Title: Structural and functional basis of phospholipid oxygenase activity of bacterial lipoxygenase from Pseudomonas aeruginosa.
Article Snippet: Structural and functional basis of phospholipid oxygenase activity of bacterial lipoxygenase from Pseudomonas aeruginosa Swathi Banthiya, Jacqueline Kalms, Etienne Galemou Yoga, Igor Ivanov, Xavi Carpena, Mats Hamberg, Hartmut Kuhn, Patrick Scheerer PII: S1388-1981(16)30222-0 DOI: doi: 10.1016/j.bbalip.2016.08.002 Reference: BBAMCB 58023 To appear in: BBA - Molecular and Cell Biology of Lipids Received date: 19 May 2016 Revised date: 29 July 2016 Accepted date: 3 August 2016 Please cite this article as: Swathi Banthiya, Jacqueline Kalms, Etienne Galemou Yoga, Igor Ivanov, Xavi Carpena, Mats Hamberg, Hartmut Kuhn, Patrick Scheerer, Structural and functional basis of phospholipid oxygenase activity of bacterial lipoxygenase from Pseudomonas aeruginosa, BBA - Molecular and Cell Biology of Lipids (2016), doi: 10.1016/j.bbalip.2016.08.002 This is a PDF file of an unedited manuscript that has been accepted for publication.. As a service to our customers we are providing this early version of the manuscript.. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form.

Article Title: Stabilization and improved activity of arachidonate 11S-lipoxygenase from proteobacterium Myxococcus xanthus
Article Snippet: HFA standards, including 11S-HETE, 11R-HETE, 15S-HETE, 15R-HETE, 11-hydroxyeicosapentaenoic acid (HEPE), and 14-hydroxydocosahexaenoic acid (HDOHE), were purchased from Cayman Chemical.

Article Title: Peroxiredoxin III Protects Tumor Suppressor PTEN from Oxidation by 15-Hydroperoxy-eicosatetraenoic Acid
Article Snippet: 15s-HpETE and 15s-HETE were purchased from Cayman Chemical (Ann Arbor, MI, USA).

Article Title: Involvement of Proinflammatory Arachidonic Acid (ARA) Derivatives in Crohn’s Disease (CD) and Ulcerative Colitis (UC)
Article Snippet: Standards of ARA and LA derivatives: Thromboxane B2 (TXB2), Prostaglandin E2 (PGE2), Leukotriene B4 (LTB4), 13S-HODE (13S-hydroxy-9Z,11E-octadecadienoic acid), 9S-HODE (9S-hydroxy-10E, 12Z-octadecadienoic acid), 15S-HETE (15S-hydroxy-5Z,8Z,11Z,13E-eicosatetraenoic acid), 12S-HETE (12S-hydroxy-5Z,8Z,10E,14Z-eicosatetraenoic acid), 5-oxoETE (5-Oxo-eicosatetraenoic acid), 5-HETE (5-hydroxyeicosatetraenoic acid), 5(S),6(R)-lipoxin-A4 (5S,6R,15S-trihydroxy-7E,9E,11Z,13E-eicosatetraenoic acid), 5(S),6(R)15(R)-lipoxin-A4, (5(S),6(R),15(R)-trihydroxy 7E,9E,11Z,13E-eicosatetraenoic acid), 16RS-HETE Cayman Chemicals (Ann Arbor, MI, USA).

Inhibition:

Article Title: The crystal structure of Pseudomonas aeruginosa lipoxygenase Ala420Gly mutant explains the improved oxygen affinity and the altered reaction specificity.
Article Snippet: The crystal structure of Pseudomonas aeruginosa lipoxygenase Ala420Gly mutant explains the improved oxygen affinity and the altered reaction specificity Jacqueline Kalms, Swathi Banthiya, Etienne Galemou Yoga, Mats Hamberg, Hermann-Georg Holzhutter, Hartmut Kuhn, Patrick Scheerer PII: S1388-1981(17)30003-3 DOI: doi:10.1016/j.bbalip.2017.01.003 Reference: BBAMCB 58101 To appear in: BBA - Molecular and Cell Biology of Lipids Received date: 25 October 2016 Revised date: 3 January 2017 Accepted date: 12 January 2017 Please cite this article as: Jacqueline Kalms, Swathi Banthiya, Etienne Galemou Yoga, Mats Hamberg, Hermann-Georg Holzhutter, Hartmut Kuhn, Patrick Scheerer, The crystal structure of Pseudomonas aeruginosa lipoxygenase Ala420Gly mutant explains the improved oxygen affinity and the altered reaction specificity, BBA - Molecular and Cell Biology of Lipids (2017), doi:10.1016/j.bbalip.2017.01.003 This is a PDF file of an unedited manuscript that has been accepted for publication.. As a service to our customers we are providing this early version of the manuscript.. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form.

Article Title: Crystal Structure of Arachidonic Acid Bound to a Mutant of Prostaglandin Endoperoxide H Synthase-1 That Forms Predominantly 11-Hydroperoxyeicosatetraenoic Acid
Article Snippet: Materials—Arachidonic acid, 11R-HETE, and 15S-HETE were pur- chased from Cayman Chemical Co. (Ann Arbor, MI).

Article Title: Synergistic effect of 15-lipoxygenase 2 and radiation in killing head-and-neck cancer.
Article Snippet: 15S-HETE and AA were purchased from Cayman Chemical Inc. (An Arbor, MI).

Article Title: Strata movement and stress evolution when mining two overlapping panels affected by hard stratum
Article Snippet: 15s-HpETE and 15s-HETE were purchased from Cayman Chemical (Ann Arbor, MI, USA).

Article Title: Structural and functional basis of phospholipid oxygenase activity of bacterial lipoxygenase from Pseudomonas aeruginosa.
Article Snippet: Structural and functional basis of phospholipid oxygenase activity of bacterial lipoxygenase from Pseudomonas aeruginosa Swathi Banthiya, Jacqueline Kalms, Etienne Galemou Yoga, Igor Ivanov, Xavi Carpena, Mats Hamberg, Hartmut Kuhn, Patrick Scheerer PII: S1388-1981(16)30222-0 DOI: doi: 10.1016/j.bbalip.2016.08.002 Reference: BBAMCB 58023 To appear in: BBA - Molecular and Cell Biology of Lipids Received date: 19 May 2016 Revised date: 29 July 2016 Accepted date: 3 August 2016 Please cite this article as: Swathi Banthiya, Jacqueline Kalms, Etienne Galemou Yoga, Igor Ivanov, Xavi Carpena, Mats Hamberg, Hartmut Kuhn, Patrick Scheerer, Structural and functional basis of phospholipid oxygenase activity of bacterial lipoxygenase from Pseudomonas aeruginosa, BBA - Molecular and Cell Biology of Lipids (2016), doi: 10.1016/j.bbalip.2016.08.002 This is a PDF file of an unedited manuscript that has been accepted for publication.. As a service to our customers we are providing this early version of the manuscript.. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form.

Article Title: Stabilization and improved activity of arachidonate 11S-lipoxygenase from proteobacterium Myxococcus xanthus
Article Snippet: HFA standards, including 11S-HETE, 11R-HETE, 15S-HETE, 15R-HETE, 11-hydroxyeicosapentaenoic acid (HEPE), and 14-hydroxydocosahexaenoic acid (HDOHE), were purchased from Cayman Chemical.

Article Title: Peroxiredoxin III Protects Tumor Suppressor PTEN from Oxidation by 15-Hydroperoxy-eicosatetraenoic Acid
Article Snippet: 15s-HpETE and 15s-HETE were purchased from Cayman Chemical (Ann Arbor, MI, USA).

Article Title: Involvement of Proinflammatory Arachidonic Acid (ARA) Derivatives in Crohn’s Disease (CD) and Ulcerative Colitis (UC)
Article Snippet: Standards of ARA and LA derivatives: Thromboxane B2 (TXB2), Prostaglandin E2 (PGE2), Leukotriene B4 (LTB4), 13S-HODE (13S-hydroxy-9Z,11E-octadecadienoic acid), 9S-HODE (9S-hydroxy-10E, 12Z-octadecadienoic acid), 15S-HETE (15S-hydroxy-5Z,8Z,11Z,13E-eicosatetraenoic acid), 12S-HETE (12S-hydroxy-5Z,8Z,10E,14Z-eicosatetraenoic acid), 5-oxoETE (5-Oxo-eicosatetraenoic acid), 5-HETE (5-hydroxyeicosatetraenoic acid), 5(S),6(R)-lipoxin-A4 (5S,6R,15S-trihydroxy-7E,9E,11Z,13E-eicosatetraenoic acid), 5(S),6(R)15(R)-lipoxin-A4, (5(S),6(R),15(R)-trihydroxy 7E,9E,11Z,13E-eicosatetraenoic acid), 16RS-HETE Cayman Chemicals (Ann Arbor, MI, USA).

Permeability:

Article Title: The crystal structure of Pseudomonas aeruginosa lipoxygenase Ala420Gly mutant explains the improved oxygen affinity and the altered reaction specificity.
Article Snippet: The crystal structure of Pseudomonas aeruginosa lipoxygenase Ala420Gly mutant explains the improved oxygen affinity and the altered reaction specificity Jacqueline Kalms, Swathi Banthiya, Etienne Galemou Yoga, Mats Hamberg, Hermann-Georg Holzhutter, Hartmut Kuhn, Patrick Scheerer PII: S1388-1981(17)30003-3 DOI: doi:10.1016/j.bbalip.2017.01.003 Reference: BBAMCB 58101 To appear in: BBA - Molecular and Cell Biology of Lipids Received date: 25 October 2016 Revised date: 3 January 2017 Accepted date: 12 January 2017 Please cite this article as: Jacqueline Kalms, Swathi Banthiya, Etienne Galemou Yoga, Mats Hamberg, Hermann-Georg Holzhutter, Hartmut Kuhn, Patrick Scheerer, The crystal structure of Pseudomonas aeruginosa lipoxygenase Ala420Gly mutant explains the improved oxygen affinity and the altered reaction specificity, BBA - Molecular and Cell Biology of Lipids (2017), doi:10.1016/j.bbalip.2017.01.003 This is a PDF file of an unedited manuscript that has been accepted for publication.. As a service to our customers we are providing this early version of the manuscript.. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form.

Article Title: Crystal Structure of Arachidonic Acid Bound to a Mutant of Prostaglandin Endoperoxide H Synthase-1 That Forms Predominantly 11-Hydroperoxyeicosatetraenoic Acid
Article Snippet: Materials—Arachidonic acid, 11R-HETE, and 15S-HETE were pur- chased from Cayman Chemical Co. (Ann Arbor, MI).

Article Title: Synergistic effect of 15-lipoxygenase 2 and radiation in killing head-and-neck cancer.
Article Snippet: 15S-HETE and AA were purchased from Cayman Chemical Inc. (An Arbor, MI).

Article Title: Strata movement and stress evolution when mining two overlapping panels affected by hard stratum
Article Snippet: 15s-HpETE and 15s-HETE were purchased from Cayman Chemical (Ann Arbor, MI, USA).

Article Title: Structural and functional basis of phospholipid oxygenase activity of bacterial lipoxygenase from Pseudomonas aeruginosa.
Article Snippet: Structural and functional basis of phospholipid oxygenase activity of bacterial lipoxygenase from Pseudomonas aeruginosa Swathi Banthiya, Jacqueline Kalms, Etienne Galemou Yoga, Igor Ivanov, Xavi Carpena, Mats Hamberg, Hartmut Kuhn, Patrick Scheerer PII: S1388-1981(16)30222-0 DOI: doi: 10.1016/j.bbalip.2016.08.002 Reference: BBAMCB 58023 To appear in: BBA - Molecular and Cell Biology of Lipids Received date: 19 May 2016 Revised date: 29 July 2016 Accepted date: 3 August 2016 Please cite this article as: Swathi Banthiya, Jacqueline Kalms, Etienne Galemou Yoga, Igor Ivanov, Xavi Carpena, Mats Hamberg, Hartmut Kuhn, Patrick Scheerer, Structural and functional basis of phospholipid oxygenase activity of bacterial lipoxygenase from Pseudomonas aeruginosa, BBA - Molecular and Cell Biology of Lipids (2016), doi: 10.1016/j.bbalip.2016.08.002 This is a PDF file of an unedited manuscript that has been accepted for publication.. As a service to our customers we are providing this early version of the manuscript.. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form.

Article Title: Stabilization and improved activity of arachidonate 11S-lipoxygenase from proteobacterium Myxococcus xanthus
Article Snippet: HFA standards, including 11S-HETE, 11R-HETE, 15S-HETE, 15R-HETE, 11-hydroxyeicosapentaenoic acid (HEPE), and 14-hydroxydocosahexaenoic acid (HDOHE), were purchased from Cayman Chemical.

Article Title: Peroxiredoxin III Protects Tumor Suppressor PTEN from Oxidation by 15-Hydroperoxy-eicosatetraenoic Acid
Article Snippet: 15s-HpETE and 15s-HETE were purchased from Cayman Chemical (Ann Arbor, MI, USA).

Article Title: Involvement of Proinflammatory Arachidonic Acid (ARA) Derivatives in Crohn’s Disease (CD) and Ulcerative Colitis (UC)
Article Snippet: Standards of ARA and LA derivatives: Thromboxane B2 (TXB2), Prostaglandin E2 (PGE2), Leukotriene B4 (LTB4), 13S-HODE (13S-hydroxy-9Z,11E-octadecadienoic acid), 9S-HODE (9S-hydroxy-10E, 12Z-octadecadienoic acid), 15S-HETE (15S-hydroxy-5Z,8Z,11Z,13E-eicosatetraenoic acid), 12S-HETE (12S-hydroxy-5Z,8Z,10E,14Z-eicosatetraenoic acid), 5-oxoETE (5-Oxo-eicosatetraenoic acid), 5-HETE (5-hydroxyeicosatetraenoic acid), 5(S),6(R)-lipoxin-A4 (5S,6R,15S-trihydroxy-7E,9E,11Z,13E-eicosatetraenoic acid), 5(S),6(R)15(R)-lipoxin-A4, (5(S),6(R),15(R)-trihydroxy 7E,9E,11Z,13E-eicosatetraenoic acid), 16RS-HETE Cayman Chemicals (Ann Arbor, MI, USA).

Enzyme-linked Immunosorbent Assay:

Article Title: The crystal structure of Pseudomonas aeruginosa lipoxygenase Ala420Gly mutant explains the improved oxygen affinity and the altered reaction specificity.
Article Snippet: The crystal structure of Pseudomonas aeruginosa lipoxygenase Ala420Gly mutant explains the improved oxygen affinity and the altered reaction specificity Jacqueline Kalms, Swathi Banthiya, Etienne Galemou Yoga, Mats Hamberg, Hermann-Georg Holzhutter, Hartmut Kuhn, Patrick Scheerer PII: S1388-1981(17)30003-3 DOI: doi:10.1016/j.bbalip.2017.01.003 Reference: BBAMCB 58101 To appear in: BBA - Molecular and Cell Biology of Lipids Received date: 25 October 2016 Revised date: 3 January 2017 Accepted date: 12 January 2017 Please cite this article as: Jacqueline Kalms, Swathi Banthiya, Etienne Galemou Yoga, Mats Hamberg, Hermann-Georg Holzhutter, Hartmut Kuhn, Patrick Scheerer, The crystal structure of Pseudomonas aeruginosa lipoxygenase Ala420Gly mutant explains the improved oxygen affinity and the altered reaction specificity, BBA - Molecular and Cell Biology of Lipids (2017), doi:10.1016/j.bbalip.2017.01.003 This is a PDF file of an unedited manuscript that has been accepted for publication.. As a service to our customers we are providing this early version of the manuscript.. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form.

Article Title: Crystal Structure of Arachidonic Acid Bound to a Mutant of Prostaglandin Endoperoxide H Synthase-1 That Forms Predominantly 11-Hydroperoxyeicosatetraenoic Acid
Article Snippet: Materials—Arachidonic acid, 11R-HETE, and 15S-HETE were pur- chased from Cayman Chemical Co. (Ann Arbor, MI).

Article Title: Synergistic effect of 15-lipoxygenase 2 and radiation in killing head-and-neck cancer.
Article Snippet: 15S-HETE and AA were purchased from Cayman Chemical Inc. (An Arbor, MI).

Article Title: Strata movement and stress evolution when mining two overlapping panels affected by hard stratum
Article Snippet: 15s-HpETE and 15s-HETE were purchased from Cayman Chemical (Ann Arbor, MI, USA).

Article Title: Structural and functional basis of phospholipid oxygenase activity of bacterial lipoxygenase from Pseudomonas aeruginosa.
Article Snippet: Structural and functional basis of phospholipid oxygenase activity of bacterial lipoxygenase from Pseudomonas aeruginosa Swathi Banthiya, Jacqueline Kalms, Etienne Galemou Yoga, Igor Ivanov, Xavi Carpena, Mats Hamberg, Hartmut Kuhn, Patrick Scheerer PII: S1388-1981(16)30222-0 DOI: doi: 10.1016/j.bbalip.2016.08.002 Reference: BBAMCB 58023 To appear in: BBA - Molecular and Cell Biology of Lipids Received date: 19 May 2016 Revised date: 29 July 2016 Accepted date: 3 August 2016 Please cite this article as: Swathi Banthiya, Jacqueline Kalms, Etienne Galemou Yoga, Igor Ivanov, Xavi Carpena, Mats Hamberg, Hartmut Kuhn, Patrick Scheerer, Structural and functional basis of phospholipid oxygenase activity of bacterial lipoxygenase from Pseudomonas aeruginosa, BBA - Molecular and Cell Biology of Lipids (2016), doi: 10.1016/j.bbalip.2016.08.002 This is a PDF file of an unedited manuscript that has been accepted for publication.. As a service to our customers we are providing this early version of the manuscript.. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form.

Article Title: Stabilization and improved activity of arachidonate 11S-lipoxygenase from proteobacterium Myxococcus xanthus
Article Snippet: HFA standards, including 11S-HETE, 11R-HETE, 15S-HETE, 15R-HETE, 11-hydroxyeicosapentaenoic acid (HEPE), and 14-hydroxydocosahexaenoic acid (HDOHE), were purchased from Cayman Chemical.

Article Title: Peroxiredoxin III Protects Tumor Suppressor PTEN from Oxidation by 15-Hydroperoxy-eicosatetraenoic Acid
Article Snippet: 15s-HpETE and 15s-HETE were purchased from Cayman Chemical (Ann Arbor, MI, USA).

Article Title: Involvement of Proinflammatory Arachidonic Acid (ARA) Derivatives in Crohn’s Disease (CD) and Ulcerative Colitis (UC)
Article Snippet: Standards of ARA and LA derivatives: Thromboxane B2 (TXB2), Prostaglandin E2 (PGE2), Leukotriene B4 (LTB4), 13S-HODE (13S-hydroxy-9Z,11E-octadecadienoic acid), 9S-HODE (9S-hydroxy-10E, 12Z-octadecadienoic acid), 15S-HETE (15S-hydroxy-5Z,8Z,11Z,13E-eicosatetraenoic acid), 12S-HETE (12S-hydroxy-5Z,8Z,10E,14Z-eicosatetraenoic acid), 5-oxoETE (5-Oxo-eicosatetraenoic acid), 5-HETE (5-hydroxyeicosatetraenoic acid), 5(S),6(R)-lipoxin-A4 (5S,6R,15S-trihydroxy-7E,9E,11Z,13E-eicosatetraenoic acid), 5(S),6(R)15(R)-lipoxin-A4, (5(S),6(R),15(R)-trihydroxy 7E,9E,11Z,13E-eicosatetraenoic acid), 16RS-HETE Cayman Chemicals (Ann Arbor, MI, USA).



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Image Search Results


The regulatory role of Alox15/15-HETE in CLP-induced SIMD . (A) Graphs showing the levels of 15-HETE in the serum of WT and Irgm1-cKO mice after CLP (n = 5 per group). (B) Graphs showing the levels of 15-HETE in the supernatants of neutrophils from WT and Irgm1-cKO mice treated with LPS (n = 5 per group). (C) Schematic illustration of 15-HETE treatment in WT mice. The mice were intraperitoneally injected with 15-HETE (0.5 mg/kg). (D) Survival analysis of WT mice given Vehicle (n = 20) or 15-HETE (n = 26) after CLP was monitored for 24h. (E) Representative M-mode echocardiograms obtained from WT mice given Vehicle or 15-HETE after CLP. Scale bars = 2 mm (vertical) and 0.1 s (horizontal). (F–I) Echocardiographic measurements of left ventricular ejection fraction (EF, F), fractional shortening (FS, G), left ventricular internal diastolic dimension (LVIDD, H), and left ventricular end-diastolic volume (LVEDV, I) from WT mice given Vehicle or 15-HETE after CLP (n = 8 per group). (J) Relative ANP and BNP mRNA expression levels of the cardiac in WT mice given Vehicle or 15-HETE after CLP. (K and L) Graphs showing the levels of cTnI (K) and LDH (L) in the serum of WT mice given Vehicle or 15-HETE after CLP (n = 5 per group). (M) Relative mRNA expression levels of cardiac inflammation-related genes in WT mice given Vehicle or 15-HETE after CLP (n = 5 per group). (N) Representative images of H&E staining and quantitative analysis of myocardial injury. Scale bars: 50 μm for high magnification, 500 μm for low magnification. (O) TUNEL staining and quantification of apoptotic cardiomyocytes in heart tissues from WT mice given Vehicle or 15-HETE after CLP (n = 5 per group). Scale bar = 20 μm. (P) Representative TEM images of heart sections from WT mice given Vehicle or 15-HETE after CLP. Scale bars: 0.5 μm for high magnification, 1 μm for low magnification. Data are represented as the mean ± SD. Log-rank test for D. Unpaired Student t -test for A, and B, F through O. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ∗∗∗∗ p < 0.0001.

Journal: Redox Biology

Article Title: Neutrophil Irgm1 ameliorates sepsis-induced myocardial dysfunction by promoting Alox15 degradation

doi: 10.1016/j.redox.2026.104104

Figure Lengend Snippet: The regulatory role of Alox15/15-HETE in CLP-induced SIMD . (A) Graphs showing the levels of 15-HETE in the serum of WT and Irgm1-cKO mice after CLP (n = 5 per group). (B) Graphs showing the levels of 15-HETE in the supernatants of neutrophils from WT and Irgm1-cKO mice treated with LPS (n = 5 per group). (C) Schematic illustration of 15-HETE treatment in WT mice. The mice were intraperitoneally injected with 15-HETE (0.5 mg/kg). (D) Survival analysis of WT mice given Vehicle (n = 20) or 15-HETE (n = 26) after CLP was monitored for 24h. (E) Representative M-mode echocardiograms obtained from WT mice given Vehicle or 15-HETE after CLP. Scale bars = 2 mm (vertical) and 0.1 s (horizontal). (F–I) Echocardiographic measurements of left ventricular ejection fraction (EF, F), fractional shortening (FS, G), left ventricular internal diastolic dimension (LVIDD, H), and left ventricular end-diastolic volume (LVEDV, I) from WT mice given Vehicle or 15-HETE after CLP (n = 8 per group). (J) Relative ANP and BNP mRNA expression levels of the cardiac in WT mice given Vehicle or 15-HETE after CLP. (K and L) Graphs showing the levels of cTnI (K) and LDH (L) in the serum of WT mice given Vehicle or 15-HETE after CLP (n = 5 per group). (M) Relative mRNA expression levels of cardiac inflammation-related genes in WT mice given Vehicle or 15-HETE after CLP (n = 5 per group). (N) Representative images of H&E staining and quantitative analysis of myocardial injury. Scale bars: 50 μm for high magnification, 500 μm for low magnification. (O) TUNEL staining and quantification of apoptotic cardiomyocytes in heart tissues from WT mice given Vehicle or 15-HETE after CLP (n = 5 per group). Scale bar = 20 μm. (P) Representative TEM images of heart sections from WT mice given Vehicle or 15-HETE after CLP. Scale bars: 0.5 μm for high magnification, 1 μm for low magnification. Data are represented as the mean ± SD. Log-rank test for D. Unpaired Student t -test for A, and B, F through O. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ∗∗∗∗ p < 0.0001.

Article Snippet: For pharmacological interventions, PD146176 (Alox15 inhibitor; 10 mg/kg, HY-103157, MedChemExpress) or 15-HETE (Arachidonic acid metabolites; 0.5 mg/kg, sc-200944, Santa Cruz Biotechnology) was administered intraperitoneally 15 min before CLP.

Techniques: Injection, Expressing, Staining, TUNEL Assay

Administration of PD146176 can eliminate the detrimental effects caused by Irgm1 deficiency in vivo . (A) Schematic illustration of PD146176 treatment in Irgm1-cKO mice. The mice were intraperitoneally injected with PD146176 (10 mg/kg). (B) Graphs showing the levels of 15-HETE in the serum of Irgm1-cKO mice given Vehicle or PD146176 after CLP (n = 5 per group). (C) Survival analysis of Irgm1-cKO mice given Vehicle (n = 24) or PD146176 (n = 18) after CLP performance was monitored for 24h. (D) Representative M-mode echocardiograms obtained from Irgm1-cKO mice given Vehicle or PD146176 after CLP. Scale bars = 2 mm (vertical) and 0.1 s (horizontal). (E-H) Echocardiographic measurements of left ventricular ejection fraction (EF, E), fractional shortening (FS, F), left ventricular internal diastolic dimension (LVIDD, G), and left ventricular end-diastolic volume (LVEDV, H) of Irgm1-cKO mice given Vehicle or PD146176 after CLP (n = 8 per group). Scale bars = 2 mm (vertical) and 0.1 s (horizontal). (I) Representative images of H&E staining and quantitative analysis of myocardial injury. Scale bars: 100 μm for high magnification, 500 μm for low magnification. (J and K) Graphs showing the levels of LDH (J) and cTnI (K) in the serum of Irgm1-cKO mice given Vehicle or PD146176 after CLP (n = 5 per group). (L) Relative ANP and BNP mRNA expression levels of the cardiac in Irgm1-cKO mice given Vehicle or PD146176 after CLP (n = 5 per group). (M) Relative mRNA expression levels of cardiac inflammation-related genes in Irgm1-cKO mice given Vehicle or PD146176 after CLP (n = 5 per group). (N) TUNEL staining and quantification of apoptotic cardiomyocytes in heart tissues from Irgm1-cKO mice given Vehicle or PD146176 after CLP (n = 5 per group). Scale bar = 20 μm. (O) Representative TEM images of heart sections from Irgm1-cKO mice given Vehicle or PD146176 after CLP. Scale bars: 0.5 μm for high magnification, 1 μm for low magnification. Data are represented as the mean ± SD. Log-rank test for C. Unpaired Student t -test for B, and E through N. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ∗∗∗∗ p < 0.0001.

Journal: Redox Biology

Article Title: Neutrophil Irgm1 ameliorates sepsis-induced myocardial dysfunction by promoting Alox15 degradation

doi: 10.1016/j.redox.2026.104104

Figure Lengend Snippet: Administration of PD146176 can eliminate the detrimental effects caused by Irgm1 deficiency in vivo . (A) Schematic illustration of PD146176 treatment in Irgm1-cKO mice. The mice were intraperitoneally injected with PD146176 (10 mg/kg). (B) Graphs showing the levels of 15-HETE in the serum of Irgm1-cKO mice given Vehicle or PD146176 after CLP (n = 5 per group). (C) Survival analysis of Irgm1-cKO mice given Vehicle (n = 24) or PD146176 (n = 18) after CLP performance was monitored for 24h. (D) Representative M-mode echocardiograms obtained from Irgm1-cKO mice given Vehicle or PD146176 after CLP. Scale bars = 2 mm (vertical) and 0.1 s (horizontal). (E-H) Echocardiographic measurements of left ventricular ejection fraction (EF, E), fractional shortening (FS, F), left ventricular internal diastolic dimension (LVIDD, G), and left ventricular end-diastolic volume (LVEDV, H) of Irgm1-cKO mice given Vehicle or PD146176 after CLP (n = 8 per group). Scale bars = 2 mm (vertical) and 0.1 s (horizontal). (I) Representative images of H&E staining and quantitative analysis of myocardial injury. Scale bars: 100 μm for high magnification, 500 μm for low magnification. (J and K) Graphs showing the levels of LDH (J) and cTnI (K) in the serum of Irgm1-cKO mice given Vehicle or PD146176 after CLP (n = 5 per group). (L) Relative ANP and BNP mRNA expression levels of the cardiac in Irgm1-cKO mice given Vehicle or PD146176 after CLP (n = 5 per group). (M) Relative mRNA expression levels of cardiac inflammation-related genes in Irgm1-cKO mice given Vehicle or PD146176 after CLP (n = 5 per group). (N) TUNEL staining and quantification of apoptotic cardiomyocytes in heart tissues from Irgm1-cKO mice given Vehicle or PD146176 after CLP (n = 5 per group). Scale bar = 20 μm. (O) Representative TEM images of heart sections from Irgm1-cKO mice given Vehicle or PD146176 after CLP. Scale bars: 0.5 μm for high magnification, 1 μm for low magnification. Data are represented as the mean ± SD. Log-rank test for C. Unpaired Student t -test for B, and E through N. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ∗∗∗∗ p < 0.0001.

Article Snippet: For pharmacological interventions, PD146176 (Alox15 inhibitor; 10 mg/kg, HY-103157, MedChemExpress) or 15-HETE (Arachidonic acid metabolites; 0.5 mg/kg, sc-200944, Santa Cruz Biotechnology) was administered intraperitoneally 15 min before CLP.

Techniques: In Vivo, Injection, Staining, Expressing, TUNEL Assay